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Differences in LTP Between Dorsomedial and Dorsolateral Striatum: Induction Frequency and D1 Receptors

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dc.contributor.advisor Blackwell, Kim T Lewitus, Valerie J
dc.creator Lewitus, Valerie J 2018-05-02 2018-08-08T20:13:21Z 2018-08-08T20:13:21Z
dc.description.abstract The striatum of the basal ganglia is involved in learning, such as the acquisition of precise motor control and habits. The dorsomedial (DM) striatum receives input from the association cortex and is involved in spatial learning and goal-oriented behavior, while the dorsolateral (DL) striatum receives input from the primary sensorimotor cortex and is involved in procedural learning and habit formation. Previous studies have found that neuron spiking is entrained to a higher TBS frequency in the DM than the DL striatum. In addition, previous studies have shown dopamine D1 receptors are required in the DM striatum and others show that these receptors are not required in the DL striatum. It is unclear whether the latter is due to a true regional difference or due to differing experimental protocols. To investigate regional differences in learning between these two areas, we tested whether the induction of LTP in the DM and DL striatum differs across different theta-burst stimulation (TBS) frequencies and in the presence or absence of D1 receptors. We recorded extracellular field potentials in adult male C57Bl/6 mouse brain slices in response to a theta burst stimulation of either 10.5 Hz or 5 Hz. We found that 10.5 Hz – but not 5 Hz – produces LTP dorsomedially; in contrast, 5 Hz – but not 10.5 Hz – produces similar LTP amplitudes dorsolaterally. To test for the requirement of D1 receptors, we blocked the receptors using SCH23390 in the DL striatum and found that this blocks LTP, which is similar to what has been found in the DM striatum. Additionally, in order to determine whether female mice are appropriate subjects to use in this study, we observed whether mouse sex or estrus status influences the ability to induce LTP using 10.5 Hz in the DM striatum. We found that female mice who are not in the estrus stage exhibit LTP of a similar amplitude to male mice, but female mice who are in the estrus stage do not exhibit LTP under the same stimulation protocol.
dc.language.iso en en_US
dc.subject long-term potentiation en_US
dc.subject dorsal striatum en_US
dc.subject basal ganglia en_US
dc.subject theta-burst stimulation en_US
dc.subject sex differences en_US
dc.subject D1 receptors en_US
dc.title Differences in LTP Between Dorsomedial and Dorsolateral Striatum: Induction Frequency and D1 Receptors en_US
dc.type Thesis en_US Master of Science in Biology en_US Master's en_US Biology en_US George Mason University en_US

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